生物来源
rabbit
质量水平
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse, chicken, rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:1000))
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pTyr1068)
基因信息
chicken ... Egfr(396494)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)
一般描述
Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1068.
Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr¹⁰⁶⁸).
免疫原
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
应用
Immunoblotting (1:1000)
警告
Toxicity: Standard Handling (A)
外形
In Dulbecco′s PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
其他说明
Jo, M., et al. 2000. J. Biol. Chem.275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.
When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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