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Merck
CN

33-036

PI 3-Kinase HTRF® Assay; 10,000 wells

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Key Documents

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Product Name

PI 3-Kinase HTRF® Assay; 10,000 wells, The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.

species reactivity

mouse, human

manufacturer/tradename

HTRF®
Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_006218.2

UniProt accession no.

P42336

detection method

fluorometric (HTRF)

shipped in

dry ice

Quality Level

100

Gene Information

human ... PIK3CA(5290)

Application

PI 3-Kinase HTRF® Assay; 10,000 wells has been used for measuring the enzymatic activity of the class I phosphoinositide (PI) 3-kinase isoforms by a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

Biochem/physiol Actions

Target Sub-Family: PI3K/PI4K

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phosphoinositide (PI) 3-kinase (PI3K) is a heterodimeric lipid kinase containing regulatory and catalytic subunits. It catalyzes the phosphorylation at the 3-position hydroxyl group of the inositol ring of phosphatidylinositol (PtdIns). PI3K is implicated in various cellular functions such as cell growth, proliferation, motility, differentiation, survival, apoptosis, cytoskeletal rearrangement, and intracellular trafficking. It also plays a vital role in glucose uptake triggered by insulin.

Other Notes

4X Reaction Buffer

PIP2 (Substrate; 1mM stock)

Stop Reagent A

Stop Reagent B

DM A Detection Reagent

DM B Detection Reagent

DM C Detection Reagent

Packaging

Kit capacity: 10,000 wells

Preparation Note

12 months at -70°C

Legal Information

HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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