EDAC, Hydrochloride

Molar absorptivity (~212 nm; 50 mM phosphate buffer, pH 8):≥6800 M^-1cm^-1.

Water-soluble carbodiimide derivative. Used to modify NMDA receptors. Also useful for conjugating haptens to proteins or polypeptides.

Water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Packaged under inert gas

Avoid amine containing buffers.

Unstable in solution. Reconstitute just prior to use.

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Kopple, K.D., et al. 1962. J. Am. Chem. Soc.84, 4457.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Irritant (B)