345806-M
β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli
Synonym(s):
β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli, β1,4-D-Galactoside Galactohydrolase
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About This Item
CAS Number:
NACRES:
NA.77
UNSPSC Code:
12352202
MDL number:
Specific activity:
≥1.5 units/mL, ≥6 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
form
liquid
specific activity
≥1.5 units/mL, ≥6 units/mg protein
packaging
pkg of 50 mIU
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
N-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
storage temp.
2-8°C
General description
Note: 1 mU = 1 milliunit.
Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Application
Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
Other Notes
Kierman, U.A., et al. 2004. Proteomincs4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 345806-50MIU | 04055977194081 |
| 345806-500MIU | 04055977194098 |