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Merck
CN

36-003

Anti-phospho-MYPT1 (Thr850) Antibody

Upstate®, from rabbit

Synonym(s):

Anti-GUBS, Anti-M130, Anti-MBS, Anti-MYPT1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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Product Name

Anti-phospho-MYPT1 (Thr850) Antibody, Upstate®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

chicken, rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pThr850)

Quality Level

Gene Information

human ... PPP1R12A(4659)

Analysis Note

Control
MYPT1 substrate phosphorylated by ROKa/ROCK-II
Routinely evaluated by immunoblot on phophorylated MYPT1 substrate

Application

Detect phospho-MYPT1 (Thr850) using this Anti-phospho-MYPT1 (Thr850) Antibody validated for use in WB.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Biochem/physiol Actions

Recombinant truncated chicken MYPT1 phosphorylated on Thr850.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

80 kDa

Immunogen

Peptide corresponding to amino acid residues 845-855 of chicken MYPT1; this sequence is identical to amino acids 848-858 of human MYPT1, with the phosphorylated Thr at position 853.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.
Protein A purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mechanically induced osteogenic differentiation--the role of RhoA, ROCKII and cytoskeletal dynamics.
Arnsdorf, EJ; Tummala, P; Kwon, RY; Jacobs, CR
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