Name: PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)
Brand: MM

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

2D3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative (antibody only)

species reactivity

mouse, human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(ELISA (0.05 µg/mL)
Immunoblotting (0.5 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer9)

Gene Information

human ... GSK3B(2932)
mouse ... Gsk3B(56637)

General description

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

Application

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

Physical form

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

Preparation Note

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

Analysis Note

Positive Control
Pervanadate-treated HepG2 (included)

Other Notes

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)