Skip to Content
Merck
CN

362185

PNGase F Glycosidase

Removes all N-linked glycans, suitable for mass spectrometry, from Elizabethkingia meningosepticum

Synonym(s):

N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
83534-39-8
EC Number:
3.5.1.52 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD00131222

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

N-Glycosidase F, Elizabethkingia meningosepticum,

biological source

bacterial (Elizabethkingia meningosepticum)

conjugate

(N-linked)

form

liquid

specific activity

≥20,000 units/mg protein
≥4500 units/mL

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

Proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Quality Level

100

Related Categories

Disclaimer

Toxicity: Standard Handling (A)

General description

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Other Notes

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service