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368067-M

Granzyme B Substrate VIII, Colorimetric

Name: Granzyme B Substrate VIII, Colorimetric
Brand: MM

A colorimetric substrate for granzyme B (kcat/Km = 6.6 x 10⁴ M⁻¹s⁻¹).

Synonym(s):

Granzyme B Substrate VIII, Colorimetric, Ac-IEPD- pNA

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About This Item

Empirical Formula (Hill Notation):

C₂₈H₃₈N₆O₁₁

Molecular Weight:

634.63

UNSPSC Code:

12352200

PubChem Substance ID:

2826717

NACRES:

NA.77

Assay:

≥95% (HPLC)

Form:

lyophilized solid

Quality level:

100

Storage condition:

OK to freeze, protect from light

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Properties

Quality Level

100

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

white

solubility

DMSO: 5 mg/mL

storage temp.

−20°C

SMILES string

CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)C

InChI key

YXEIIOVAGGAMCZ-UHFFFAOYSA-N

Description

General description

A colorimetric substrate for granzyme B (k_cat/K_m = 6.6 x 10⁴ M^-1s^-1). Cleavage of pNA is monitored colorimetrically at ~405 nm (ε = 9,160 M^-1cm^-1).

Biochem/physiol Actions

Primary Target
Colorimetric substrate for granzyme B

kcat/Km = 6.6 x 104 M⁻¹ sec⁻¹ as substrate for granzyme B

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ac-Ile-Glu-Pro-Asp-pNA

Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c