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Merck
CN

373273-M

Hh Signaling Antagonist XIV, SANT-2

The Hh Signaling Antagonist XIV, SANT-2, also referenced under CAS 329196-48-7, controls the biological activity of Hh pathway. This small molecule/inhibitor is primarily used for Cell Signaling applications.

Synonym(s):

Hh Signaling Antagonist XIV, SANT-2, N-[3-(1H-Benzimidazol-2-yl)-4-chlorophenyl]-3,4,5-tris(ethyloxy)benzamide, Smo Antagonist V

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About This Item

Empirical Formula (Hill Notation):
C26H26ClN3O4
CAS Number:
329196-48-7
Molecular Weight:
479.96
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
powder
Storage condition:
OK to freeze, protect from light

Key Documents

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storage temp.

2-8°C

form

powder

InChI key

VQOJFGFKIVFMDH-UHFFFAOYSA-N

InChI

1S/C26H26ClN3O4/c1-4-32-22-13-16(14-23(33-5-2)24(22)34-6-3)26(31)28-17-11-12-19(27)18(15-17)25-29-20-9-7-8-10-21(20)30-25/h7-15H,4-6H2,1-3H3,(H,28,31)(H,29,30)

assay

≥95% (HPLC)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

white

solubility

DMSO: 100 mg/mL, ethanol: 25 mg/mL

General description

A nanomolar Smoothened (smo) antagonist that exhibits 2.3- and 6-fold weaker biological activity than SANT-1 (Cat. No. 559303 ), respectively, in antagonizing ShhNp- and SAG-stimulated hedgehog pathway activation in HEK 293-based (Shh-LIGHT2) reporter assays. Competitive binding studies show full replacement of Smo-bound cyclopamine (Cat. No. 239803) by SANT-1 and SANT-2 (Ki = 2.4 and 8.4 nM, respectively), while SANT-1 and SANT-2 are shown to compete against Smo-bound SAG-1.3 (Cat. Nos. 566660 & 566661) in an allosteric manner and that only SANT-2, but not SANT-1, is able to completely compete off bound SAG-1.3 (100% vs. 40% replacement by the respective compound at 10 µM).

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Rominger, C.M., et al. 2009. J. Pharmacol. Exp. Ther.329, 995.
Rohatgi, R., et al. 2009. Proc. Natl. Acad. Sci. USA106, 3196.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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