382065
Bisbenzimide H 33342 Fluorochrome, Trihydrochloride
Cell-permeable, adenine-thymine-specific fluorescent stain.
Synonym(s):
Bisbenzimide H 33342 Fluorochrome, Trihydrochloride, 2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
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About This Item
Empirical Formula (Hill Notation):
C27H28N6O · 3HCl
CAS Number:
Molecular Weight:
561.93
MDL number:
UNSPSC Code:
12352111
NACRES:
NA.21
Quality Level
description
RTECS - DT4200000
assay
≥95% (TLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
yellow
solubility
water: 50 mg/mL
fluorescence
λex 346 nm, λem 460 nm
shipped in
ambient
storage temp.
2-8°C
SMILES string
N1(CCN(CC1)c2cc3nc([nH]c3cc2)c4cc5nc([nH]c5cc4)c6ccc(cc6)OCC)C
InChI
1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31)
InChI key
PRDFBSVERLRRMY-UHFFFAOYSA-N
General description
Cell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.
Cell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Adenine-thymine-specific fluorescent stain
Adenine-thymine-specific fluorescent stain
Product does not compete with ATP.
Reversible: no
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol.118, 148.
Sandhu, L.C., et al. 1985. Cytometry6, 191.
Frankfurt, O.S. 1983. Cytometry4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA78, 363.
Visser, J.W.M. 1980. Flow CytometryIV, 86.
Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
Belloc, F., et al. 1994. Cytometry17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
Hinkley, R.E., et al. 1986. Dev. Biol.118, 148.
Sandhu, L.C., et al. 1985. Cytometry6, 191.
Frankfurt, O.S. 1983. Cytometry4, 216.
Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA78, 363.
Visser, J.W.M. 1980. Flow CytometryIV, 86.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Toxic & Carcinogenic / Teratogenic (G)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Karl Kerns et al.
International journal of molecular sciences, 21(6) (2020-03-25)
Building on our recent discovery of the zinc signature phenomenon present in boar, bull, and human spermatozoa, we have further characterized the role of zinc ions in the spermatozoa's pathway to fertilization. In boar, the zinc signature differed between the
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Integrins, the extracellular matrix receptors that facilitate cell adhesion and migration, are necessary for organ morphogenesis; however, their role in maintaining adult tissue homeostasis is poorly understood. To define the functional importance of β1 integrin in adult mouse lung, we
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 382065-100MG | 04055977213089 |