382129
HIV Reverse Transcriptase, Recombinant, E. coli
liquid, ≥5,000 units/mg protein, Nuclease-free
Synonym(s):
HIV RT
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352202
Form:
liquid
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
form
liquid
specific activity
≥10,000 units/mL, ≥5,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
molecular cloning: suitable
input
purified RNA
foreign activity
Nuclease-free
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.
Application
HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 382129-500U | 04055977213140 |