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382159-M

PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

Name: PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

Clone:

polyclonal

Species reactivity:

Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans

Application:

—

Citations:

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Properties

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

isotype

IgG

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer10)

Gene Information

human ... H3C1(8350)

Description

General description

Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

This PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).

Immunogen

Human

a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH

Application

Immunoblotting (0.5 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunohistochemistry (see comments)

Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Acid-extracted proteins from colcemid-arrested HeLa cells

Other Notes

Ajiro, K., et al. 1996. J. Biol. Chem.271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.

For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c