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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

M16.10A1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, porcine, chicken, canine, human, hamster, guinea pig, bovine, rat, monkey, sheep

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA
Enzyme Immunoassay
Immunoblotting (1 µg/mL, colorimetric)
Immunocytochemistry (1 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation (5 µg/mL)
Paraffin Sections )

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SERPINH1(871)

General description

Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
Recognizes the ~47 kDa Hsp47 protein.

Immunogen

Hsp47 from L6 cells
Rat

Application

ELISA (see comments)

Enzyme Immunoassay (see comments)

Immunoblotting (1 µg/ml, colorimetric)

Immunocytochemistry (1 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Paraffin Sections (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
3T3 cells, Human breast cancer tissue

Other Notes

Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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