Merck
CN
All Photos(1)

Documents

420320

Sigma-Aldrich

KT5720

A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (Ki = 56 nM) that is prepared by a chemical modification of K-252a.

Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
KT5720, PKA Inhibitor VII
Empirical Formula (Hill Notation):
C32H31N3O5
CAS Number:
108068-98-0
Molecular Weight:
537.61
MDL number:
MFCD00132118

Quality Level

100

Assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white

solubility

DMSO: 1 mg/mL

shipped in

ambient

storage temp.

−20°C

InChI

1S/C32H31N3O5/c1-3-4-5-10-15-39-30(37)32(38)16-23-34-21-13-8-6-11-18(21)25-26-20(17-33-29(26)36)24-19-12-7-9-14-22(19)35(28(24)27(25)34)31(32,2)40-23/h6-9,11-14,23,38H,3-5,10,15-17H2,1-2H3,(H,33,36)/t23-,31+,32+/m0/s1

InChI key

ZHEHVZXPFVXKEY-RUAOOFDTSA-N

General description

A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (Ki = 56 nM) that is prepared by a chemical modification of K-252a. Does not significantly affect the activities of PKC, PKG, and MLCK. Inhibits axon branching in cultured neurons. A 2 mM (50 µg/47 µl) solution of KT 5720 (Cat. No. 420323) in DMSO is also available.
Prepared by a chemical modification of K-252a. A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (Ki = 56 nM). Does not significantly affect the activity of PKC, PKG, or MLCK. Inhibits axon branching in cultured neurons.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
PKA
Product competes with ATP.
Reversible: yes
Target Ki: 56 nM for protein kinase A

Warning

Toxicity: Standard Handling (A)

Preparation Note

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Simpson, C.S., and Morris, B.J. 1995. Neuroscience 68, 97.
Cabell, L., and Audesirk, G. 1993. Int. J. Dev. Neurosci.11, 357.
Gadbois, D.M., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8626.
Kase, H., et al. 1987. Biochem. Biophys. Res. Commun.142, 436.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Tomomi Kurashige et al.
Journal of the Endocrine Society, 4(7), bvaa054-bvaa054 (2020-07-17)
Autophagy is an evolutionarily conserved catabolic process by which cells degrade intracellular proteins and organelles in the lysosomes and recycle their metabolites. We have recently demonstrated the crucial role for the basal level of autophagic activity in thyrocyte survival and
Kyohei Tokizane et al.
Glia, 65(5), 740-755 (2017-02-10)
Under a quiescent state, microglia exhibit a ramified shape, rather than the amoeboid-like morphology following injury or inflammation. The manipulation of microglial morphology in vitro has not been very successful, which has impeded the progress of microglial studies. We demonstrate

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service