420411
Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested
An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code.
Synonym(s):
Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested
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About This Item
Empirical Formula (Hill Notation):
C18H36N4O11 · xH2SO4
CAS Number:
Molecular Weight:
484.50 (free base basis)
UNSPSC Code:
12352200
Form:
solid
Storage condition:
OK to freeze
Quality Segment
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white to off-white
solubility
water: 10 mg/mL
shipped in
ambient
storage temp.
10-30°C
InChI
1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
InChI key
OOYGSFOGFJDDHP-KMCOLRRFSA-N
General description
Recommended working concentration: 100 µg/ml.
An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity. Useful for the selection of kanamycin resistant plasmids in E. coli. Tested and found to be negative for cytotoxicity against HeLa cells. Contains more than 98% kanamycin A.
Contains >98% kanamycin A. Potency: ≥750 µg/mg
Biochem/physiol Actions
Primary Target
protein biosynthesis that acts on the 30S ribosome
protein biosynthesis that acts on the 30S ribosome
Preparation Note
Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
Other Notes
Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Cardon, G.H., et al. 1993. Plant J.3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother.6, 516.
Umezawa, S., et al. 1968. J. Antibiot.21, 367.
Cardon, G.H., et al. 1993. Plant J.3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother.6, 516.
Umezawa, S., et al. 1968. J. Antibiot.21, 367.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Carcinogenic / Teratogenic (D)
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
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