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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
biological source
Bacillus sp. (Bacillus cereus)
Quality Level
description
Merck USA index - 14, 7093
form
lyophilized
packaging
vial of ≥50 units β-lactamase II
vial of ≥500 units β-lactamase I
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
sterile distilled water: soluble
shipped in
ambient
storage temp.
−20°C
General description
β-Lactamase produced by bacteria shares sequence homology to penicillin-binding proteins. This enzyme is found in Gram-negative bacteria. β-Lactamases have four molecular classes, named A, B, C, and D. A, C, and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes, which have a zinc ion at their active site for β-lactam hydrolysis.
Biochem/physiol Actions
β-Lactamase hydrolyzes β-lactum antibiotics and is the chief cause of resistance to β-lactam antibiotics development by bacteria. Mutations in the β-lactamases are associated with extended-spectrum β-lactamases (ESBLs).
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 month at 4°C.
Other Notes
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol benzyl penicillin and 1.0 µmol of cephalosporin C, respectively, per min at 25°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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