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About This Item
Empirical Formula (Hill Notation):
C12H19NO3S
CAS Number:
Molecular Weight:
257.35
MDL number:
UNSPSC Code:
12161902
NACRES:
NA.21
Quality Level
Assay
≥99% (TLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
color
white
solubility
water: 200 mg/mL
storage temp.
10-30°C
SMILES string
[S](=O)(=O)([O-])CCC[N+](Cc1ccccc1)(C)C
InChI
1S/C12H19NO3S/c1-13(2,9-6-10-17(14,15)16)11-12-7-4-3-5-8-12/h3-5,7-8H,6,9-11H2,1-2H3
InChI key
MEJASPJNLSQOAG-UHFFFAOYSA-N
General description
A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.
A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.
Preparation Note
Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature.
Other Notes
Goldberg, M.E., et al. 1996. Folding & Design1, 21.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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