Assay
≥80% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥75 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−70°C
General description
Full-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Harrington, L.S., et al. 2005. Trends Biochem. Sci.30, 35.
Osaki, M., et al. 2004. Apoptosis9, 667.
Lennon, G., et al. 1996. Genomics33, 151.
Osaki, M., et al. 2004. Apoptosis9, 667.
Lennon, G., et al. 1996. Genomics33, 151.
One unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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