481409
PTEN, His-Tag® and S-Tag, Human Recombinant, S. frugiperda
别名:
Phosphatase and Tensin Homolog Deleted on Chromosome 10
assay
≥80% (SDS-PAGE)
form
liquid
specific activity
≥75 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Full-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Harrington, L.S., et al. 2005. Trends Biochem. Sci.30, 35.
Osaki, M., et al. 2004. Apoptosis9, 667.
Lennon, G., et al. 1996. Genomics33, 151.
Osaki, M., et al. 2004. Apoptosis9, 667.
Lennon, G., et al. 1996. Genomics33, 151.
One unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持