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484235

p-Nitroblue Tetrazolium Chloride

≥98% (TLC), crystalline solid, nitric oxide synthase inhibitor, Calbiochem®

Synonym(s):

p-Nitroblue Tetrazolium Chloride, NBT, Nitro BT

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About This Item

Empirical Formula (Hill Notation):
C40H30Cl2N10O6
CAS Number:
298-83-9
Molecular Weight:
817.64
UNSPSC Code:
12171500
NACRES:
NA.77
MDL number:
MFCD00012159
Assay:
≥98% (TLC)
Form:
crystalline solid
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
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Product Name

p-Nitroblue Tetrazolium Chloride, NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM).

Quality Segment

100

description

RTECS - XF8045000

assay

≥98% (TLC)

form

crystalline solid

potency

3-4 μM IC50

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

yellow

solubility

methanol: soluble, water: soluble

shipped in

ambient

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].[n+]2([n](nc(n2)c8ccccc8)c3c(cc(cc3)c4cc(c(cc4)[n]5[n+](nc(n5)c7ccccc7)c6ccc(cc6)[N+](=O)[O-])OC)OC)c1ccc(cc1)[N+](=O)[O-]

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

General description

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).

Biochem/physiol Actions

Primary Target
NOS

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.

Other Notes

Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis.16, 29.
Libon, C., et al. 1993. J. Leukoc. Biol.53, 93.
Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
Nichols, K., et al. 1992. Neuroscience51, 791.
Nichols, K., et al. 1992. Neuroscience51, 791.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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