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Merck
CN

48-632MAG

MILLIPLEX® Total/Phospho Akt Signaling Panel

Premixed Akt2 Total/Phospho 2-Plex Cell Signaling Panel

Synonym(s):

Akt2 ELISA, Akt2 assay, Akt2 kinase assay, Akt2 multiplex, Akt2 phosphoprotein panel, Akt2 phosphorylation, PI3K-Akt2 signaling, phospho-Akt2, total Akt2

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About This Item

NACRES:
NA.47
UNSPSC Code:
12161503
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Quality Level

species reactivity

rat, mouse, human

technique(s)

multiplexing: suitable

input

cell lysate

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

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General description

This panel targets phosphorylated and total Akt2, an isoform critical for insulin signaling and glucose metabolism. Akt2 (pS474) is activated in response to insulin and plays a key role in metabolic homeostasis. Dysregulation of Akt2 is associated with insulin resistance and type 2 diabetes.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 2 analytes in human, mouse, or rat cell lysate samples.

Analytes Included: Akt2 (Phospho-Ser474), Akt2 (Total)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate Control Assays).

Features and Benefits

  • Simultaneously detect phosphorylated and total Akt2 in a single well
  • Includes Akt2 (Phospho-Ser474) and Akt2 (Total)
  • Magnetic bead-based format ensures reproducibility and efficient processing
  • Compatible with cell lysates from insulin signaling, metabolism, and cancer studies
  • Ideal for isoform-specific analysis in metabolic and oncology research
  • Fast, convenient alternative to Western Blotting and immunoprecipitation procedures

Legal Information

Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Regulatory Information

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Related Content

Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.

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