Skip to Content
Merck
CN

48-680MAG

MILLIPLEX® Multi-Pathway Signaling Panel

Premixed Multi-Pathway Phosphoprotein 9-Plex Cell Signaling Panel

Synonym(s):

ERK Cell Signaling Assay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay, MAPK STAT TCR assay, multi-pathway signaling panel, multi-target phosphorylation panel, phosphoprotein multiplex assay, rat cell signaling panel, rat intracellular signaling biomarkers

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

MILLIPLEX® Multi-Pathway Signaling Panel, Premixed Multi-Pathway Phosphoprotein 9-Plex Cell Signaling Panel

Quality Level

200

species reactivity

rat, mouse, human

manufacturer/tradename

Milliplex®

packaging

1 ea of

technique(s)

multiplexing: suitable

input

cell lysate

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Application

Research Category
Cell Signaling
Research Sub Category
Oncology
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 9 analytes in human, mouse, or rat cell lysate samples.

Analytes Included: CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (Thr180/Tyr182), p70S6K (Thr412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Preparation Note

Recommended storage for kit components is 2 - 8°C

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

  • Simultaneously detect 9 phosphorylated proteins across multiple signaling pathways
  • Covers key pathways including MAPK, STAT, Akt, and NFκB
  • Magnetic bead-based format ensures reproducibility and efficient processing
  • Compatible with cell lysates from diverse experimental conditions
  • Ideal for signal transduction, oncology, immunology, and toxicology research
  • Fast, convenient alternative to Western Blotting and immunoprecipitation procedures

General description

This panel includes phosphorylated proteins involved in multiple signaling pathways that regulate cell survival, proliferation, inflammation, and transcriptional activity. CREB (pS133) is a transcription factor activated by cAMP signaling. ERK (pT185/pY187), JNK (pT183/pY185), and p38 (Thr180/Tyr182) are key components of the MAPK pathway. Akt (pS473) regulates cell survival and metabolism. NFκB (pS536) controls inflammatory and immune responses. p70S6K (Thr412) is involved in protein synthesis, while STAT3 (pS727) and STAT5A/B (pY694/699) mediate cytokine signaling and immune regulation

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Christopher P Stanley et al.
Cardiovascular research, 107(4), 568-578 (2015-06-21)
The protective effects of cannabidiol (CBD) have been widely shown in preclinical models and have translated into medicines for the treatment of multiple sclerosis and epilepsy. However, the direct vascular effects of CBD in humans are unknown. Using wire myography
William H Hind et al.
British journal of pharmacology, 173(5), 815-825 (2015-10-27)
In vivo and in vitro studies have demonstrated a protective effect of cannabidiol (CBD) in reducing infarct size in stroke models and against epithelial barrier damage in numerous disease models. We aimed to investigate whether CBD also affects blood-brain barrier
Paolo Carpineto et al.
Investigative ophthalmology & visual science, 57(15), 6895-6901 (2016-12-22)
To evaluate signal transduction and early apoptosis protein levels in subretinal fluid collected during scleral buckling surgery for macula-off rhegmatogenous retinal detachment (RRD). Our aim was to assess both their relation with RRD features and their influence on the posttreatment
Paul S Hafen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 125(5), 1447-1455 (2018-07-20)
The heat stress response is associated with several beneficial adaptations that promote cell health and survival. Specifically, in vitro and animal investigations suggest that repeated exposures to a mild heat stress (~40°C) elicit positive mitochondrial adaptations in skeletal muscle comparable
Belinda I Gómez et al.
The Journal of surgical research, 248, 28-37 (2019-12-17)
The endothelial glycocalyx controls vascular permeability, cellular signaling, blood-endothelial cell adhesion, extravasation, and transmission of shear stress signals. Burn injury compromises integrity of this layer increasing vascular permeability, which is further exacerbated by large volumes of (intravenous) crystalloids. We have
View All Related Papers

Related Content

Overview of Luminex® Multiplex Assay Technology | MILLIPLEX® Multiplex Assays

Discover the benefits of MILLIPLEX® multiplex assays, based on Luminex® xMAP® multiplex assay technology, that provide consistent, high-quality results and see how these multiplex biomarker Luminex® assays are being used to advance research.

Multiplexing Cancer & Metastasis Signaling Pathways | MILLIPLEX® Assays

Learn how multiplexing with MILLIPLEX® multiplex assays can help simplify cancer research by measuring multiple circulating biomarkers at once. Find multiplex panels to analyze JAK-STAT, mTOR, MAPK, SAPK, TGFβ signaling pathways, and more.

Multiplexing Inflammation Signaling Pathways | MILLIPLEX® Assays

See how multiplexing the inflammation signaling pathway with MILLIPLEX® inflammation assays or cell signaling assays can help researchers bridge the gap between immunology and cell signaling, including investigating T cell signaling, Th Cell differentiation, inflammatory response signaling, and sepsis signaling.

Cell Signaling Multiplex Assays | MILLIPLEX® Assays

Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service