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Merck
CN

48-690MAG

MILLIPLEX® T-Cell Receptor Signaling Panel

Premixed T-Cell Receptor Phosphoprotein 7-Plex Cell Signaling Panel

Synonym(s):

7-Plex Magnetic Bead Kit, Magnetic Bead Kit, T-Cell Receptor Signaling Kit, T-cell phosphoprotein assay, T-cell receptor multiplex assay, TCR phosphorylation detection, TCR signaling panel, adaptive immunity signaling biomarkers, rat immune activation panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Key Documents

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Product Name

MILLIPLEX® T-Cell Receptor Signaling Panel, Premixed T-Cell Receptor Phosphoprotein 7-Plex Cell Signaling Panel

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

packaging

1 ea of

technique(s)

multiplexing: suitable

input

cell lysate

assay range

inter-assay cv: 4-17%
intra-assay cv: 3-8%

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Preparation Note

Recommended storage for kit components is 2 - 8°C

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

  • Simultaneously detect 7 phosphorylated proteins involved in T-cell receptor signaling
  • Includes CD3ζ, LAT, Lck, ZAP70, PLCγ1, SLP-76, and ERK for comprehensive immune profiling
  • Magnetic bead-based format ensures reproducibility and efficient processing
  • Compatible with cell lysates from T-cell activation and immunotherapy studies
  • Ideal for adaptive immunity, immune modulation, and cell signaling research
  • Fast, convenient alternative to Western Blotting and immunoprecipitation procedures

General description

This panel includes phosphorylated proteins central to T-cell receptor signaling and immune activation. CD3ζ (pY142) initiates TCR signaling upon antigen recognition. LAT (pY191) and SLP-76 (pY128) serve as scaffolding proteins for downstream signaling. Lck (pY394) and ZAP70 (pY319) are tyrosine kinases that propagate TCR signals. PLCγ1 (pY783) mediates calcium signaling, while ERK (pT185/pY187) links TCR activation to gene expression and cell proliferation.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 7 analytes in cell lysate samples.

Analytes Included: CD3ζ (pY142), LAT (pY191), Lck (pY394), PLCγ1 (pY783), ZAP70 (pY319), SLP-76 (pY128), ERK (pT185/pY187)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Regulatory Information

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Cell Signaling Multiplex Assays | MILLIPLEX® Assays

Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.

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