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Merck
CN

4876

p-Nitrophenyl Phosphate, disodium salt, hexahydrate

≥99% (HPLC), crystalline solid, alkaline phosphatase-based ELISA assay substrate, Calbiochem®

Synonym(s):

p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, pNPP, 2Na

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About This Item

Empirical Formula (Hill Notation):
C6H4NO6P · 2Na · 6H2O
CAS Number:
4264-83-9
Molecular Weight:
371.14
UNSPSC Code:
41116158
NACRES:
NA.77
MDL number:
MFCD00007319

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Product Name

p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM).

SMILES string

[Na+].[Na+].[P](=O)([O-])([O-])Oc1ccc(cc1)[N+](=O)[O-]

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

assay

≥99% (HPLC)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

impurities

≤0.005% p-nitrophenol (weight), ≤1% free phosphate (weight)

color

light yellow

solubility

water: 5 mg/mL

shipped in

ambient

Quality Level

100

Related Categories

General description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate for alkaline phosphatase-based ELISA assays
Product does not compete with ATP.
Reversible: no

Preparation Note

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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