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487655

p-Nitrophenyl Phosphate, DiTris Salt

Name: <i>p</i>-Nitrophenyl Phosphate, DiTris Salt
Brand: MM

Excellent substrate for alkaline phosphatase-based ELISA assays.

Synonym(s):

p-Nitrophenyl Phosphate, DiTris Salt, pNPP, 2Tris

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₂₈N₃O₁₂P

CAS Number:

68189-42-4

Molecular Weight:

461.36

UNSPSC Code:

41116158

NACRES:

NA.77

MDL number:

MFCD00037009

Assay:

≥98% (HPLC)

Form:

solid

Storage condition:

OK to freeze

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Properties

Quality Segment

100

assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

impurities

≤0.05% Free-p-Nitrophenol

color

off-white to pale yellow

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

SMILES string

[P](=O)([O-])([O-])Oc1ccc(cc1)[N+](=O)[O-].[N+H3]C(CO)(CO)CO.[N+H3]C(CO)(CO)CO

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

Description

General description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate for alkaline phosphatase-based ELISA assays

Product does not compete with ATP.

Reversible: no

Preparation Note

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c