5.08605
Sodium Orthovanadate, Activated
Synonym(s):
Sodium Orthovanadate, Activated, Orthovanadate, Activated, Vanadate, Activated
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About This Item
Empirical Formula (Hill Notation):
Na3O4V
Molecular Weight:
183.91
NACRES:
NB.21
UNSPSC Code:
12352202
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
color
colorless
storage temp.
−70°C
Quality Level
Biochem/physiol Actions
Primary Target
Phosphatases
Phosphatases
Reversible: yes
Disclaimer
Toxicity: Toxic (F)
General description
A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.
Other Notes
Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.
Packaging
Packaged under inert gas
Physical form
Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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