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5.09227

G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested

Name: G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested
Brand: MM

Synonym(s):

G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested, G418, G 418

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

CAS Number:

108321-42-2

Molecular Weight:

692.71

UNSPSC Code:

12352203

MDL number:

MFCD00058314

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Properties

assay

≥98% (TLC)

Quality Level

200

form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, protect from light

storage temp.

2-8°C

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

Description

General description

The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml

Packaging

Packaged under inert gas

Physical form

Sterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in PBS, pH 7.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

Other Notes

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

pictograms

GHS08

signalword

Danger

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_c

Not applicable

flash_point_f