Key Documents

View All Documentation

5.09228

Y-27632

Name: Y-27632
Brand: MM

≥95% (HPLC), liquid, Rho kinase inhibitor, Calbiochem

Synonym(s):

InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₁₄H₂₁N₃O · 2HCl

CAS Number:

146986-50-7

Molecular Weight:

320.26

MDL number:

MFCD18829832

UNSPSC Code:

12352200

NACRES:

NA.77

Assay:

≥95% (HPLC)

Form:

liquid

Quality level:

100

Storage condition:

OK to freeze, avoid repeated freeze/thaw cycles, protect from light

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Y-27632, InSolution, ≥95%, 10 mM aqueous solution, Sterile-Filtered, inhibitor of Rho-associated protein kinases

Quality Level

100

assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, protect from light

storage temp.

−20°C

SMILES string

N[C@@H](C1CCC(CC1)C(=O)Nc2ccncc2)C

InChI

1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11?,12?/m1/s1

InChI key

IYOZTVGMEWJPKR-VOMCLLRMSA-N

Description

General description

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 in H₂O.

Ready to use, cell culture-tested, suitable for stem cell applications. Acts as a highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160ROCK). Also shown to improve survival of human stem cells especially during cell culture passage and manipulation.

Application

InSolution Y-27632, Sterile-Filtered, Cell Culture-Tested, CAS 146986-50-7, is a 10 mM sterile-filtered solution of Y-27632 in H₂O. Acts as an inhibitor of Rho kinases (Ki = 140 nM for p160ROCK).

Biochem/physiol Actions

Primary Target
ROCK

Packaging

Packaged under inert gas

Physical form

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 (Cat. No. 688000) in H₂O.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Other Notes

Li X. et al, et. Al., 2009. Human Reproduction, 24, 580.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J.351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1999. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science.285, 895.
Uehata, M., et al. 1997. Nature.389, 990.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c