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Merck
CN

524625

Phosphatase Inhibitor Cocktail II

liquid, for the inhibition of acid phosphatases, alkaline phosphatases and protein tyrosine phosphatases (PTP's). Suitable for use with cell lysates and tissue extracts.

Synonym(s):

Phosphatase Inhibitor, Phosphatase Inhibitor Solution (Set II)

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C

Key Documents

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Product Name

Phosphatase Inhibitor Cocktail Set II, A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Related Categories

Application

Phosphatase Inhibitor Cocktail Set II has been used:
  • in triple detergent buffer to lyse cultured cells to extract total protein for western blots
  • as a component of lysis buffer to lyse tissue samples or cultured rat ventricular cardiomyocytes (VCMs) for western blot analysis of cardiac mitochondrial proteins
  • as a component of lysis buffer to lyse cultured rat VCMs infected with Adv-COX7RP and Adv-shRNA COX7RP for immunoprecipitation of ryanodine receptors type 2 (RyR2)
  • as a component in ice-cold RIPA buffer to homogenize/lyse retina tissues for protein extraction and western blotting

Biochem/physiol Actions

Protein phosphorylation and dephosphorylation play essential roles in signal transduction processes that regulate various cellular functions including cell division, proliferation, and apoptosis. Protein kinases transfer phosphate groups to specific serine, threonine, or tyrosine residues, leading to a conformational change in the protein and affecting its interaction with ligands. Protein phosphatases reverse this process by removing the phosphate group and restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain the phosphorylation state of the proteins intact.

Disclaimer

Toxicity: Standard Handling (A)

Features and Benefits

  • Flexible: compatible with cell lysates and tissue extracts, including samples containing detergents.
  • Economical: ready-to-use format minimizes wastage.
  • Effective: one ml is adequate for approximately 100 ml extraction buffer.
  • Complete: contains five phosphatase inhibitors targeting acid and alkaline phosphatases and protein tyrosine phosphatases (PTPs).

General description

A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Provided as a set of five 1 ml vials containing the following components:
  • Imidazole (200 mM)
  • Sodium Fluoride (100 mM)
  • Sodium Molybdate (115 mM)
  • Sodium Orthovanadate (100 mM)
  • Sodium Tartrate Dihydrate (400 mM)      

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes
Clements RT, et al.
Basic Research in Cardiology, 118, 1-22 (2023)
Rapamycin and fasting sustain autophagy response activated by ischemia reperfusion injury and promote retinal ganglion cell survival
Russo R, et al.
Cell Death & Disease, 9, 1-18 (2018)
The Endolysosomal Transporter DMT1 is Required for Morphine Regulation of Neuronal Ferritin Heavy Chain
Irollo E, et al.
Journal of Neuroimmune Pharmacology, 18(3), 495-508 (2023)
Aurore Thomazeau et al.
Molecular psychiatry (2020-07-02)
Many neurodevelopmental disorders are characterized by impaired functional synaptic plasticity and abnormal dendritic spine morphology, but little is known about how these are related. Previous work in the Fmr1-/y mouse model of fragile X (FX) suggests that increased constitutive dendritic
Danyang Song et al.
Acta biochimica et biophysica Sinica, 53(9), 1216-1226 (2021-08-08)
Glycosylation is a common posttranslational modification of proteins, which plays a role in the malignant transformation, growth, progression, chemoresistance, and immune response of tumors. Disulfide isomerase family A3 (PDIA3) specifically acts on newly synthesized glycoproteins to promote the correct folding
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