recombinant
expressed in insect cells (S. frugiperda)
Quality Level
form
liquid
specific activity
≥0.25 U/μL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Full-length, recombinant, human phosphodiesterase 4D3 (accession no. L20970) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation.
Physical form
In 150 mM NaCl, 10 mM Tris, pH 8.3 .
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Xu, R.X., et al. 2000. Science288, 1822.
Nemoz, G., et al. 1996. FEBS Lett.384, 97.
Xu, R.X., et al. 2000. Science288, 1822.
Nemoz, G., et al. 1996. FEBS Lett.384, 97.
One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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