recombinant
expressed in insect cells (S. frugiperda)
form
liquid
specific activity
≥0.25 U/μL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Full-length, recombinant, human phosphodiesterase 4D3 (accession no. L20970) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation.
Physical form
In 150 mM NaCl, 10 mM Tris, pH 8.3 .
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Xu, R.X., et al. 2000. Science288, 1822.
Nemoz, G., et al. 1996. FEBS Lett.384, 97.
Xu, R.X., et al. 2000. Science288, 1822.
Nemoz, G., et al. 1996. FEBS Lett.384, 97.
One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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