524751-M
Phosphodiesterase 7A1, Human, Recombinant, S. frugiperda
Synonym(s):
hPDE7A1
recombinant
expressed in baculovirus infected Sf21 cells
Quality Level
form
liquid
specific activity
≥0.5 U/μL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
storage temp.
−70°C
General description
Full-length, recombinant, human phosphodiesterdase 7A1 (PDE7A1) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE7A1 is required for T-cell activation induced by the costimulation of CD3 and CD28 receptors. Increased PDE7A1 correlates with a decrease in cAMP, increased IL-2, and increased cell proliferation. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Physical form
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Han, P., et al. 2006. J. Biol. Chem.281, 15050.
Lugnier, C. 2006. Pharmacol Ther.109, 366.
Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.
Lugnier, C. 2006. Pharmacol Ther.109, 366.
Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.
One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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