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Merck
CN

525200-M

Phospholipase D, Streptomyces chromofuscus

Synonym(s):

Phospholipase D, Streptomyces chromofuscus, PLD

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About This Item

CAS Number:
9001-87-0
EC Number:
3.1.4.4 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD00131897
Specific activity:
≥50 units/mg dry wt.

Key Documents

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form

lyophilized solid

specific activity

≥50 units/mg dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

color

brownish

solubility

10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON™ X-100: 1 mg/mL

foreign activity

Aatalase, glucose oxidase, none detected

storage temp.

−20°C

Quality Level

100

General description

Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.
Kurz, T., et al. 1993. Circ. Res. 72, 701.
Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Harmful (C)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Information

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