53702
Pronase® Protease, Streptomyces griseus
Synonym(s):
Pronase® powder
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About This Item
Specific activity:
≥45,000 proteolytic units/g dry wt
description
RTECS - UK9595000
form
lyophilized
specific activity
≥45,000 proteolytic units/g dry wt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
solubility
water: 10 mg/mL
shipped in
ambient
storage temp.
2-8°C
Quality Level
Legal Information
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
General description
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
Note: 1 KU = 1000 units.
Other Notes
Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
Physical form
Lyophilized from a solution containing calcium acetate as a stabilizer.
Preparation Note
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
Certificates of Analysis (COA)
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