540221
Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli
Synonym(s):
PNP
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About This Item
NACRES:
NA.42
UNSPSC Code:
12352202
Specific activity:
≥25 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
form
liquid
specific activity
≥25 units/mg protein
manufacturer/tradename
Calbiochem®
shipped in
dry ice
storage temp.
−20°C
General description
Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.
Physical form
In 50 mM Tris-HCl, pH 7.6.
Analysis Note
Single band by SDS-PAGE
Other Notes
Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.
One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of Pi into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
监管及禁止进口产品
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Roman S Iwasaki et al.
Nucleic acids research, 48(17), e101-e101 (2020-08-17)
Recent efforts in biological engineering have made detection of nucleic acids in samples more rapid, inexpensive and sensitive using CRISPR-based approaches. We expand one of these Cas13a-based methods to detect small molecules in a one-batch assay. Using SHERLOCK-based profiling of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 540221-1MG | 04055977268751 |