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Merck
CN

550701-M

Pyruvate Oxidase, Aerococcus viridans

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

Synonym(s):

Pyruvate Oxidase, Aerococcus viridans

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About This Item

CAS Number:
9001-96-1
EC Number:
1.2.3.3 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD00132148
Specific activity:
≥25 U/mg

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form

lyophilized solid

specific activity

≥25 U/mg

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

impurities

≤0.006% GPT

color

yellow

pI 

4.0

solubility

aqueous buffer: soluble

foreign activity

GOT and lactate oxidase ≤0.002%, catalase ≤0.3%

storage temp.

−20°C

Quality Level

100

General description

Native pyruvate oxidase from Aerococcus viridans. Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hager, L.P. and Lipmann, F. 1955. Methods Enzymol. 1, 482.
One unit is defined as the amount of enzyme which generates 1 µmol of H₂O₂ per min at 37°C, pH 6.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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