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551826

Sigma-Aldrich

QUIN 2, Tetrapotassium Salt

Binding of Ca2+ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield.

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Synonym(s):
QUIN 2, Tetrapotassium Salt, 2-{[2- bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8- bis(carboxymethyl)aminoquinoline, 4K, 2-{[2-bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8-bis(carboxymethyl)aminoquinoline, 4K
Empirical Formula (Hill Notation):
C26H23N3O10 · 4K
CAS Number:
73630-23-6
Molecular Weight:
693.87
MDL number:
MFCD00065487

Quality Level

100

Assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

water: 10 mg/mL

fluorescence

λex 332 nm (high)
λex 354 nm (low)
λem 505 nm (high)
λem 510 nm (low)

shipped in

ambient

storage temp.

−20°C

InChI

1S/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4

InChI key

XAGUNWDMROKIFJ-UHFFFAOYSA-J

Related Categories

General description

Binding of Ca2+ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.
Binding of Ca2+ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).

Biochem/physiol Actions

Cell permeable: no
Primary Target
Calcium Probes
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.
Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.
Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.
Bryant, D.T. 1985. Biochem. J.226, 613.
Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.
Ashley R.H., et al. 1984. Biochem. J.219, 149.
Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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