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Merck
CN

551826

QUIN 2, Tetrapotassium Salt

Binding of Ca2+ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield.

Synonym(s):

QUIN 2, Tetrapotassium Salt, 2-{[2- bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8- bis(carboxymethyl)aminoquinoline, 4K, 2-{[2-bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8-bis(carboxymethyl)aminoquinoline, 4K

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About This Item

Empirical Formula (Hill Notation):
C26H23N3O10 · 4K
CAS Number:
73630-23-6
Molecular Weight:
693.87
UNSPSC Code:
12352302
NACRES:
NA.25
MDL number:
MFCD00065487
Assay:
≥95% (HPLC)
Form:
solid
Solubility:
water: 10 mg/mL

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Product Name

QUIN 2, Tetrapotassium Salt, Binding of Ca2+ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield.

SMILES string

[K+].[K+].[K+].[K+].N(CC(=O)[O-])(CC(=O)[O-])c1c(cc(cc1)C)OCc2nc3c(cc(cc3N(CC(=O)[O-])CC(=O)[O-])OC)cc2

InChI

1S/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4

InChI key

XAGUNWDMROKIFJ-UHFFFAOYSA-J

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

yellow

solubility

water: 10 mg/mL

fluorescence

λex 332 nm (high)
λex 354 nm (low)
λem 505 nm (high)
λem 510 nm (low)

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: no
Primary Target
Calcium Probes
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Irritant (B)

General description

Binding of Ca2+ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.
Binding of Ca2+ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).

Other Notes

Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.
Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.
Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.
Bryant, D.T. 1985. Biochem. J.226, 613.
Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.
Ashley R.H., et al. 1984. Biochem. J.219, 149.
Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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