553514-M
RAD51 Inhibitor II, RI-1
The RAD51 Inhibitor, RI-1 controls the biological activity of RAD51. This small molecule/inhibitor is primarily used for Cell Structure applications.
Synonym(s):
RAD51 Inhibitor II, RI-1, 3-chloro-1-(3,4-dichlorophenyl)-4-morpholino-1H-pyrrole-2,5-dione
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About This Item
Empirical Formula (Hill Notation):
C14H11Cl3N2O3
Molecular Weight:
361.61
UNSPSC Code:
12352200
NACRES:
NA.28
Assay:
≥99% (HPLC)
Form:
solid
Storage condition:
OK to freeze, protect from light
Quality Segment
assay
≥99% (HPLC)
form
solid
potency
5-30 μM IC50
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
bright yellow
solubility
DMSO: 50 mg/mL
storage temp.
2-8°C
General description
A cell-permeable 1H-Pyrrole-2,3-dione derivative that covalently binds to RAD51 at Cys319 and irreversibly destabilizes a protein-protein interface that is essential for filament formation and recombinase activity. Stimulates single strand annealing at a 5-30 µM concentration. Inhibits subnuclear RAD51 foci formation following DNA damage without affecting replication protein A. Also, effectively sensitizes cancer cells to mitomycin C (~25 µM) and generate synergistic cell kill in RAD51C mutant cells and with lesser effect on RAD51 complemented cells. Satisfies all four Lipinski rules for predicting drug likeness.
Biochem/physiol Actions
Primary Target
RAD51
RAD51
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Budke, B., et al. 2012. Nucleic Acids Res.40, 7347.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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