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565758

β-Secretase Substrate IV, Fluorogenic

Name: &#946;-Secretase Substrate IV, Fluorogenic
Brand: MM

≥95% (HPLC), lyophilized solid, β-secretase substrate, Calbiochem^®

Synonym(s):

β-Secretase Substrate IV, Fluorogenic, FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic

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About This Item

Empirical Formula (Hill Notation):

C₉₁H₁₂₉N₂₅O₂₅S

CAS Number:

310427-94-2

Molecular Weight:

2005.22

MDL number:

MFCD03093396

NACRES:

NA.77

UNSPSC Code:

12352202

Assay:

≥95% (HPLC)

Form:

lyophilized solid

Quality level:

100

Storage condition:

OK to freeze, protect from light

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Properties

Product Name

β-Secretase Substrate IV, Fluorogenic, A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).

Quality Level

100

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

red

solubility

DMSO: 5 mg/mL

fluorescence

λ_ex ~350 nm, λ_em ~490 nm

shipped in

ambient

storage temp.

−20°C

Description

General description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported K_cat/k_m values (min^-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu^28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).

Biochem/physiol Actions

Cell permeable: no

Primary Target
β-secretase substrate

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.

H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c