565834
sFRP-3, His•Tag®, Mouse, Recombinant
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
assay
≥95% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1 EU/μg Endotxin
shipped in
ambient
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
Proliferation Assay:
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.
Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Disclaimer
Toxicity: Standard Handling (A)
General description
Recombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
Other Notes
Hoang, et al. 1996. J. Biol. Chem.271, 26131.Leyns, et al. 1997 Cell88, 747.Wang, et al. 1997 Cell88, 757.Mayr, et al. 1997 Mech. Dev.63, 109.Rattner, et al. 1997. PNAS94, 2859.
Physical form
Lyophilized from 0.2 µm filtered PBS, BSA.
Preparation Note
Following reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 2
Regulatory Information
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