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Sigma-Aldrich

Normal Goat Serum, Lyophilized Solid

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form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Normal carrier sera, produced from pooled lots of sera from healthy, non-immunized goats. Suitable for use in radioimmunoassay.

Application

Normal Goat Serum, Lyophilized Solid has been used as a blocking agent in:
  • immunohistochemistry
  • immunocytochemistry
  • immunofluorescence

Biochem/physiol Actions

Normal goat serum can be used as an alternative to commonly used sera such as fetal bovine serum (FBS) and newborn calf serum (NBCS) for the primary culture of cells from various animals. In addition, goat serum also has the potential to use for routine culture of fish cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lipid extracted, dialyzed against 250 mM NaCl, 10 mM sodium phosphate, 0.05% sodium azide, pH 7.6, and lyophilized.

Reconstitution

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Undiluted stock solutions are stable for several weeks at 4°C or for up to 1 year at -20°C. Avoid freeze/thaw cycles. Do not stored diluted stock solutions for more than 1 day at 4°C.
Reconstitute in 10 ml of H₂O. If the solution is not clear after 1-2 h standing at room temperature, centrifuge and transfer the supernatant to clean tubes.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302 + H312 + H332 - H412

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Regulatory Information

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P K Nanda et al.
In vitro cellular & developmental biology. Animal, 45(3-4), 148-151 (2009-01-02)
Serum from goat, calf, and chicken sources were evaluated in terms of attachment, growth, and proliferation of explants of Indian major carp, Cirrhinus mrigala. The attachment of explants viz. heart, liver, and kidney was directly proportional to the concentration of
Hsuan-Miao Liu et al.
International journal of molecular medicine, 41(3), 1455-1462 (2018-01-13)
Liver injury is associated with devastating consequences caused by inflammation and apoptosis. The farnesoid X receptor (FXR) is a nuclear receptor that has an essential role in hepatoprotection by maintaining the homeostasis of liver metabolism. The present study investigated the capacity
Xin Zhao et al.
International journal of molecular medicine, 42(3), 1283-1294 (2018-06-15)
Tumor necrosis factor‑related apoptosis‑inducing ligand (TRAIL) is known to induce cell apoptosis in many types of cancer cells. However, some malignant cells still exhibit anti‑apoptosis features induced by TRAIL; thus the underlying mechanisms that regulate sensitivity and resistance of tumor
Muhammet Volkan Bulbul et al.
Asian Pacific journal of cancer prevention : APJCP, 22(6), 1745-1751 (2021-06-29)
Cell culture studies are an indispensable tools used to understand basic physiological, biophysical and biomolecular mechanisms. Although traditional two-dimensional (2D) cell culture models are more preferred in experimental studies, three-dimensional (3D) cell culture models, attract more attention due to several

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