biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
9A6, monoclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr418)
General description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 (human, rat), Tyr423 (mouse), and Tyr416 (chicken, v-Src).
Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src.
This PhosphoDetect Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Src (pTyr⁴¹⁸).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src
Application
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1 µg/ml, immunofluorescence)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1 µg/ml, immunofluorescence)
Physical form
Lyophilized from 2X PBS, PEG, sucrose.
Preparation Note
Reconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Other Notes
Does not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr418), mouse (Tyr423), rat (Tyr418), chicken (Tyr416), and c-Src (Tyr416). Variables associated with assay conditions will dictate the optimal working dilution.
Martin, G.S. 2001. Nat. Rev. Mol. Cell Biol.2, 467.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol.127, 226.
Bjorge, J.D., et al. 2000. Oncogene19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods246, 203.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol.127, 226.
Bjorge, J.D., et al. 2000. Oncogene19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods246, 203.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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