569372
PhosphoDetect Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6)
lyophilized, clone 9A6, Calbiochem®
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352203
Clone:
9A6, monoclonal
Species reactivity:
human, rat, mouse
Application:
—
Citations:
-
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
9A6, monoclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr418)
Quality Level
General description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 (human, rat), Tyr423 (mouse), and Tyr416 (chicken, v-Src).
Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src.
This PhosphoDetect Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Src (pTyr⁴¹⁸).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src
Application
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1 µg/ml, immunofluorescence)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1 µg/ml, immunofluorescence)
Physical form
Lyophilized from 2X PBS, PEG, sucrose.
Preparation Note
Reconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Other Notes
Does not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr418), mouse (Tyr423), rat (Tyr418), chicken (Tyr416), and c-Src (Tyr416). Variables associated with assay conditions will dictate the optimal working dilution.
Martin, G.S. 2001. Nat. Rev. Mol. Cell Biol.2, 467.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol.127, 226.
Bjorge, J.D., et al. 2000. Oncogene19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods246, 203.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol.127, 226.
Bjorge, J.D., et al. 2000. Oncogene19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods246, 203.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
未找到合适的产品？
试试我们的产品选型工具.
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持