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574591

Sigma-Aldrich

Superoxide Dismutase, Human, Recombinant, E. coli

Synonym(s):

5-LO, Cu/Zn SOD, Human, Recombinant, E. coli

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in E. coli

Quality Level

100

Assay

≥90% (SDS-PAGE)

form

solid

specific activity

≥3000 U/mg

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

soluble (Reconstitute in sterile MQ water to a final concentration of >=100 µg/ml. Further dilute in aqueous buffers just prior to use.)

shipped in

wet ice

storage temp.

−20°C

General description

Recombinant, human superoxide dismutase expressed in E. coli as a homodimer consisting of 2 identical, 154-amino acid chains. Purified by proprietary chromatographic methods. Superoxide dismutase is an essential component of biological defense against the toxic effects of oxygen. It catalyzes the dismutation of superoxide radicals to molecular oxygen and H2O2 and plays a role in cellular aging due to its reduced activity in aging cells. Mutant forms of Cu/Zn SOD are associated with neurodegenerative disease.

Warning

Toxicity: Standard Handling (A)

Unit Definition

≥3000 units/mg.

Physical form

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Reconstitution

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 2 weeks at 4°C or for up to 4 months at -20°C.
Reconstitute in sterile MQ water to a final concentration of ≥100 µg/ml. Further dilute in aqueous buffers just prior to use.

Other Notes

Baumber, J. and Ball, B.A. 2005. Am. J. Vet. Res.66, 1415.
Dimitrova, A.A., et al. 2005. Folia Med. (Plovdiv)47, 42.
Jebara, S., et al. 2005. J. Plant Physiol.162, 929.
Mila-Kierzenkowska, C., et al. 2005. Acta Biol. Hung.56, 399.
Miszalski, Z., et al. 2005. J. Plant Physiol.162, 895.
Paramonov, B.A., et al. 2005. Bull. Exp. Biol. Med.139, 56.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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