574597-M
Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb
liquid, Calbiochem®
Synonym(s):
Anti-SOD
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About This Item
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, rat, human, monkey
Application:
—
Citations:
-
biological source
sheep
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, rat, human, monkey
manufacturer/tradename
Calbiochem®
availability
not available in Canada,
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
storage temp.
−20°C
target post-translational modification
unmodified
General description
Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.
Sheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.
Superoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O2-) to molecular oxygen (O2) and hydrogen peroxide (H2O2).
This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.
Immunogen
Human
highly purified, human erythrocyte SOD Cu/Zn.
Application
Immunoblotting (1:1000)
Immunoelectrophoresis (see comments)
Frozen sections (see comments)
Paraffin sections (see comments)
Free-floating sections (see comments)
Immunoelectrophoresis (see comments)
Frozen sections (see comments)
Paraffin sections (see comments)
Free-floating sections (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In glycine-buffered saline, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Human red blood cell lysate
Human red blood cell lysate
Other Notes
Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15763.
This antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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