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Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade

Name: Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade
Brand: MM

Sterile Solution. Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade

Synonym(s):

Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol

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About This Item

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

UNSPSC Code:

12161705

NACRES:

NA.25

MDL number:

MFCD00004679

Beilstein/REAXYS Number:

741883

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Product Name

Tris Buffer, 100 mM, pH 7.4, Molecular Biology Grade,

grade

Molecular Biology

Quality Level

100

description

Merck USA index - 14, 9772

form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

concentration

100 mM

color

clear colorless

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

foreign activity

DNases, RNases, none detected

shipped in

ambient

storage temp.

10-30°C

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

General description

Sterile solution. Widely used biological buffer. Suitable for DNA and RNA applications. Useful in the pH range of 7.0-9.0.

Widely used biological buffer. Prepared with diethylpyrocarbonate-treated water for molecular biology applications. Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Application

Structural Characterization of Toxicologically Relevant Cd(2+)-L-Cysteine Complexes.: This study utilized Tris buffer, 100 mM, pH 7.4, for the structural analysis of cadmium-cysteine complexes, providing insights into their biochemical behavior and toxicological impacts (Gautam et al., 2023).

Cation and buffer specific effects on the DNA-lipid interaction.: The research employed Tris buffer, 100 mM, pH 7.4, to explore the specific effects of cations and buffers on DNA-lipid interactions, highlighting its importance in biophysical and biochemical studies (Mura et al., 2023).

Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications.: The research utilized Tris buffer, 100 mM, pH 7.4, in the preparation of cerium-containing nanoparticles, demonstrating their potential in biomedical applications without the need for drugs (Kurtuldu et al., 2021).

Physical form

A sterile, 100 mM solution, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c