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Merck
CN

684274

β1,2-Xylosidase, Xanthomonas sp.

Synonym(s):

β1,2-Xylosidase, Xanthomonas sp., β-D-Xylanxylohydrolase

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About This Item

CAS Number:
9025-53-0
EC Number:
3.2.1.37 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
MFCD00130640
Specific activity:
≥10 units/mL, ≥20 units/mg protein

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form

liquid

specific activity

≥10 units/mL, ≥20 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

foreign activity

β-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases, none detected

shipped in

wet ice

Quality Level

100

Related Categories

General description

Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.
Note: 1 mU = 1 milliunit.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Preparation Note

Following initial use, refrigerate (4°C).

Other Notes

One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.
Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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