69522
T7•Tag® Monoclonal Antibody
Synonym(s):
T7 Tag Antibody
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Clone:
monoclonal
Species reactivity:
mouse
Application:
—
Citations:
-
clone
monoclonal
Quality Level
species reactivity
mouse
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG2b
shipped in
dry ice, wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
The T7-Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as PEPscreen® and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select® Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
Biochem/physiol Actions
T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates
Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
Working dilution- 1:5,000–1:10,000
Cross-reactivity: Negligible with bacterial, insect or mammalian cell lysates
Sensitivity: 1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
Working dilution- 1:5,000–1:10,000
Packaging
Plastic ampoule
Physical form
Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Other Notes
- Krek, W., et al. 1994. Cell 78,, 161.
- Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.
- Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PEPscreen is a registered trademark of Sigma-Aldrich Co. LLC
T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany
T7SELECT is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Multiple Toxicity Values, refer to MSDS
Regulatory Information
新产品
This item has
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 69522-4 | 07790788052584 |