pT7Blue^™ T-Vector - Novagen

The pT7Blue^™ T-Vector is designed for simplified cloning of PCR products. Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3′ A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue^™ vector has been specifically constructed for this application and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3′ dT residues at each end. The T-Vector is provided ready to ligate with DNA amplified with any DNA polymerase that leaves single 3′ dA overhangs. Target inserts having other types of ends, such as reaction products of proofreading polymerase or restriction fragments, can be easily prepared for T-cloning with the Single dA<TMSYMBOL></TMSYMBOL> Tailing Kit. pT7Blue^™ contains the pUC19 backbone (including high-copy number origin or replication and lac sequences), a T7 promoter, f1 origin of replication, and a modified multiple cloning region.

This product is only available in Japan.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

PT7BLUE is a trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)

This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the “EMD Product”) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.