pBAC-3 DNA - Novagen

pBAC-3 is a baculovirus transfer plasmid compatible with BacVector-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag and/or S•Tag sequences. pBAC-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.

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This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the “EMD Product”) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.

Toxicity: Standard Handling (A)